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Publications

Original articles

de Juan A, Tabtim-On D, Coillard A, Becher B, Goudot C, Segura E. (2024) The aryl hydrocarbon receptor shapes monocyte transcriptional responses to interleukin-4 by prolonging STAT6 binding to promoters. Sci Signal. 17(858):eadn6324
https://doi.org/10.1126/scisignal.adn6324
Open access, click here.

Villar J, Ouaknin L, Cros A, Segura E. (2023) Monocytes differentiate along two alternative pathways during sterile inflammation. EMBO Reports. e56308
https://doi.org/10.15252/embr.202256308

Cros A, De Juan A, Leclere R, Sampaio JL, San Roman M, Maurin M, Heurtebise-Chrétien S, Segura E (2023) Homeostatic activation of aryl hydrocarbon receptor by dietary ligands dampens cutaneous allergic responses by controlling Langerhans cells migration. eLife. 12:e86413
https://doi.org/10.7554/eLife.86413

Alaoui L, Villar J, Leclere R, Le Gallou S, Relouzat F, Michaud HA, Tarte K, Teissier N, Favier B, Roussel M, Segura E. (2023) Functional specialization of short-lived and long-lived macrophage subsets in human tonsils. J Exp Med. 3;220(7):e20230002.
https://doi.org/10.1084/jem.20230002

Villar J, Cros A, De Juan A, Alaoui L, Bonte PE, Lau CM, Tiniakou I, Reizis B, Segura E. (2022) ETV3 and ETV6 enable monocyte differentiation into dendritic cells by repressing macrophage fate commitment. Nat Immunol. 24: 84-95.
https://doi.org/10.1038/s41590-022-01374-0

Coillard A, Guyonnet L, De Juan A, Cros A, Segura E. (2021). TLR or NOD receptor signaling skews monocyte fate decision via distinct mechanisms driven by mTOR and miR-155. PNAS 118 (43) e2109225118
https://doi.org/10.1073/pnas.2109225118

Moutel S, Beugnet A, Schneider A, Lombard B, Loew D, Amigorena S, Perez P, Segura E. (2020). Surface LSP-1 is a phenotypic marker distinguishing human classical versus monocyte-derived dendritic cells iScience 23,100987
https://doi.org/10.1016/j.isci.2020.100987

Durand M, Walter T, Pirnay T, Naessens T, Gueguen P, Goudot C, Lameiras S, Chang Q, Talaei N, Ornatsky O, Vassilevskaia T, Baulande S, Amigorena S, Segura E. (2019). Human lymphoid organ cDC2 and macrophages play complementary roles in T follicular helper responses. J Exp Med 216(7): 1561-1581.
https://doi.org/10.1084/jem.20181994

Tang-Huau TL, Gueguen P, Goudot C, Durand M, Bohec M, Baulande S, Pasquier B, Amigorena S, Segura E (2018). Human in vivo-generated monocyte-derived dendritic cells and macrophages cross-present antigens through a vacuolar pathway. Nature Communications 9, 2570. 10.1038/s41467-018-04985-0
https://doi.org/10.1038/s41467-018-04985-0

Goudot C, Coillard A, Villani AC, Gueguen P, Cros A, Sarkizova S, Tang-Huau TL, Bohec M, Baulande S, Hacohen N, Amigorena S, Segura E (2017). Aryl hydrocarbon receptor controls monocyte differentiation into dendritic cells versus macrophages. Immunity. 47(3):582-596.
https://doi.org/10.1016/j.immuni.2017.08.016

Review articles

Rigamonti A, Villar J, Segura E (2023). Monocyte differentiation within tissues: a renewed outlook. Trends Immunol. 44(12):999-1013.
https://doi.org/10.1016/j.it.2023.10.005

Segura E (2022). Human dendritic cell subsets: an updated view of their ontogeny and functional specialization. Eur J Immunol. 52: 1759-1767.
https://doi.org/10.1002/eji.202149632

De Juan A and Segura E (2021). Modulation of Immune Responses by Nutritional Ligands of Aryl Hydrocarbon Receptor. Front. Immunol. 12:1948.
https://doi.org/10.3389/fimmu.2021.645168

Coillard A and Segura E (2021). Antigen presentation by mouse monocyte-derived cells: Re-evaluating the concept of monocyte-derived dendritic cells. Molecular Immunology. 135:165-169.
https://doi.org/10.1016/j.molimm.2021.04.012

Villar J and Segura E (2020). Decoding the Heterogeneity of Human Dendritic Cell Subsets. Trends Immunol. 41(12):1062-1071.
https://doi.org/10.1016/j.it.2020.10.002

Villar J and Segura E (2020). Recent advances towards deciphering human dendritic cell development. Molecular Immunology. 122:109-115
https://doi.org/10.1016/j.molimm.2020.04.004

Coillard A and Segura E (2019). In vivo Differentiation of Human Monocytes. Front. Immunol. 10:1907.
https://doi.org/10.3389/fimmu.2019.01907

Tang-Huau TL and Segura E (2018). Human in vivo-differentiated monocyte-derived dendritic cells. Semin Cell Dev Biol.S1084-9521(17)30362-2.
https://doi.org/10.1016/j.semcdb.2018.02.018

Durand M and Segura E (2015). The known unknowns of the human dendritic cell network. Front. Immunol. 6:129.
https://doi.org/10.3389/fimmu.2015.00129

Opinion articles

Villar J, Segura E. (2020) The More, the Merrier: DC3s Join the Human Dendritic Cell Family. Immunity. 53(2):233-235
https://doi.org/10.1016/j.immuni.2020.07.014

Segura E. (2020) Cross-dressed cDC1s instruct T cells in allorecognition. Immunology & Cell Biology. 98:520–523.
https://doi.org/10.1111/imcb.12361

Segura E, Soumelis V. (2017) Of Human DC Migrants and Residents. Immunity. 46(3):342-344.
https://doi.org/10.1016/j.immuni.2017.03.006

Guilliams M, Ginhoux F, Jakubzick C, Naik SH, Onai N, Schraml BU, Segura E, Tussiwand R, Yona S (2014). Dendritic cells, monocytes and macrophages: a unified nomenclature based on ontogeny. Nat Rev Immunol. 14(8):571-8.
https://doi.org/10.1038/nri3712

Book chapters and protocols

Villar J, Coillard A, van Roessel C, Segura E. (2023) Culture System Allowing the Simultaneous Differentiation of Human Monocytes into Dendritic Cells and Macrophages Using M-CSF, IL-4, and TNF-α. Methods Mol Biol.2618:147-154.
https://doi.org/10.1007/978-1-0716-2938-3_11

Coillard A, Tang-Huau TL, Segura E. (2023) Assessing the Ability of Human Dendritic Cells to Stimulate Naive CD4+ and CD8+ T Cells. Methods Mol Biol.2618:265-278.
https://doi.org/10.1007/978-1-0716-2938-3_19

Lutz MB, Ali S, Audiger C, Autenrieth SE, Berod L, Bigley V, Cyran L, Dalod M, Dörrie J, Dudziak D, Flórez-Grau G, Giusiano L, Godoy GJ, Heuer M, Krug AB, Lehmann CHK, Mayer CT, Naik SH, Scheu S, Schreibelt G, Segura E, Seré K, Sparwasser T, Tel J, Xu H, Zenke M. (2022) Guidelines for mouse and human DC generation. Eur J Immunol.
https://doi.org/10.1002/eji.202249816

Alaoui L, Durand M and Segura E. (2022) Identification of Antigen Presenting Cell subsets supporting human Tfh differentiation. Methods in Molecular Biology Series. 2380:125-139.
https://doi.org/10.1007/978-1-0716-1736-6_11

Coillard A and Segura E. (2018). Visualization of RNA at the Single Cell Level by Fluorescent in situ Hybridization Coupled to Flow Cytometry. Bio-protocol 8(12): e2892.
https://doi.org/10.21769/BioProtoc.2892

Segura E. (2016) Review of Mouse and Human Dendritic Cell Subsets. Methods in Molecular Biology Series. 1423:3-15
https://doi.org/10.1007/978-1-4939-3606-9_1

Durand M and Segura E. (2016) Dendritic cell subsets purification from human tonsils and lymph nodes. Methods in Molecular Biology Series. 1423:89-99
https://doi.org/10.1007/978-1-4939-3606-9_6

Segura E. (2016) Cross-presentation assay for human dendritic cells. Methods in Molecular Biology Series. 1423:189-98
https://doi.org/10.1007/978-1-4939-3606-9_14

Collaborations

Chauvin C, Alvarez-Simon D, Radulovic K, Boulard O, Laine W, Delacre M, Waldschmitt N, Segura E, Kluza J, Chamaillard M, Poulin LF. (2023) NOD2 in monocytes negatively regulates macrophage development through TNFalpha. Front Immunol. 14:1181823.
https://doi.org/10.3389/fimmu.2023.1181823

Gros M, Segura E, Rookhuizen DC, Baudon B, Heurtebise-Chrétien S, Burgdorf N, Maurin M, Kapp EA, Simpson RJ, Kozik P, Villadangos JA, Bertrand MJM, Burbage M, Amigorena S. (2022) Endocytic membrane repair by ESCRT-III controls antigen export to the cytosol during antigen cross-presentation Cell Reports. 40(7):111205
https://doi.org/10.1016/j.celrep.2022.111205

Tkach M, Thalmensi J, Timperi E, Gueguen P, Névo N, Grisard E, Sirven P, Cocozza F, Gouronnec A, Martin-Jaular L, Jouve M, Delisle F, Manel N, Rookhuizen DC, Guerin CL, Soumelis V, Romano E, Segura E, Théry C. (2022) Extracellular vesicles from triple negative breast cancer promote pro-inflammatory macrophages associated with better clinical outcome. Proc Natl Acad Sci U S A. 119(17):e2107394119.
https://doi.org/10.1073/pnas.2107394119

Liu H, Wilson KR, Firth AM, Macri C, Schriek P, Blum AB, Villar J, Wormald S, Shambrook M, Xu B, Lim HJ, McWilliam HEG, Hill AF, Edgington-Mitchell LE, Caminschi I, Lahoud MH, Segura E, Herold MJ, Villadangos JA, Mintern JD. (2022) Ubiquitin-like protein 3 (UBL3) is required for MARCH ubiquitination of major histocompatibility complex class II and CD86. Nat Commun. 13(1):1934.
https://doi.org/10.1038/s41467-022-29524-w

Kumar S, Fonseca VR, Ribeiro F, Basto AP, Agua-Doce A, Monteiro M, Elessa D, Miragaia RJ, Gomes T, Piaggio E, Segura E, Gama-Carvalho M, Teichmann SA, Graca L. (2021) Developmental bifurcation of human T follicular regulatory cells. Sci. Immunol. 6(59):eabd8411.
https://doi.org/10.1126/sciimmunol.abd8411

Erra Díaz F, Ochoa V, Merlotti A, Dantas E, Mazzitelli I, Gonzalez Polo V, Sabatté J, Amigorena S, Segura E, Geffner J. (2020). Extracellular Acidosis and mTOR Inhibition Drive the Differentiation of Human Monocyte-Derived Dendritic Cells. Cell Rep. 31(5):107613.
https://doi.org/10.1016/j.celrep.2020.107613

Michea P, Noël F, Zakine E, Czerwinska U, Sirven P, Abouzid O, Goudot C, Scholer-Dahirel A, Vincent-Salomon A, Reyal F, Amigorena S, Guillot-Delost M, Segura E, Soumelis V. (2018). Adjustment of dendritic cells to the breast-cancer microenvironment is subset specific. Nature Immunology. 19(8):885–897.
https://doi.org/10.1038/s41590-018-0145-8

Cepika AM, Banchereau R, Segura E, Ohouo M, Cantarel B, Goller K, Cantrell V, Ruchaud E, Gatewood E, Nguyen P, Gu J, Anguiano E, Zurawski S, Baisch JM, Punaro M, Baldwin N, Obermoser G, Palucka K, Banchereau J, Amigorena S, Pascual V.(2017) A multidimensional blood stimulation assay reveals immune alterations underlying systemic juvenile idiopathic arthritis. J Exp Med. 214(11):3449-3466.
https://doi.org/10.1084/jem.20170412

De Monte A, Olivieri CV, Vitale S, Bailleux S, Castillo L, Giordanengo V, Maryanski JL, Segura E, Doglio A. (2016) CD1c-Related DCs that Express CD207/Langerin, but Are Distinguishable from Langerhans Cells, Are Consistently Present in Human Tonsils. Front Immunol. 7:197.
https://doi.org/10.3389/fimmu.2016.00197

Desch AN, Gibbings SL, Goyal R, Kolde R, Bednarek J, Bruno T, Slansky JE, Jacobelli J, Mason R, Ito Y, Messier E, Randolph GJ, Prabagar M, Atif SM, Segura E, Xavier RJ, Bratton DL, Janssen WJ, Henson PM, Jakubzick CV.(2016) Flow Cytometric Analysis of Mononuclear Phagocytes in Nondiseased Human Lung and Lung-Draining Lymph Nodes. Am J Respir Crit Care Med. 193(6):614-26.
https://doi.org/10.1164/rccm.201507-1376OC

Mintern JD, Macri C, Chin WJ, Panozza SE, Segura E, Patterson NL, Zeller P, Bourges D, Bedoui S, McMillan PJ, Idris A, Nowell CJ, Brown A, Radford KJ, Johnston AP, Villadangos JA. (2015) Differential use of autophagy by primary dendritic cells specialized in cross-presentation. Autophagy. 11(6):906-17.
https://doi.org/10.1080/15548627.2015.1045178

Reuter A, Panozza SE, Macri C, Dumont C, Li J, Liu H, Segura E, Vega-Ramos J, Gupta N, Caminschi I, Villadangos JA, Johnston AP, Mintern JD. (2015) Criteria for dendritic cell receptor selection for efficient antibody-targeted vaccination. J Immunol. 194(6):2696-705.
https://doi.org/10.4049/jimmunol.1402535